Electropherogram quality analysis - Help
This suite of program analyse the sequences generated by the automatic
sequencer and gives the quality using
Phred ("Reads DNA sequencer trace data,
calls bases, assigns quality values to the bases, and writes the base
calls and quality values to output files") package using a web
The files to be analised should be "zipped" (*.zip format). For windows
based systen use 'winzip'
program to zip de files. For Linux system use a 'zip' command.
The user can see the quality at a glance (see example)
The vectors used for
screening is updated monthly from NCBI ftp site or a specific
vector sequence can be added by the system administrator.
Selecting a group of sequences, the user can run a
CAP3 program (A
DNA Sequence Assembly Program).
Any comment or suggestion e-mail to:
The following sequence is an example with the output coloured by the quality range.
At the end is shown an eletropherogram for this read:
|__|| quality lower than 10|
|__|| quality between 10 and 20|
|__|| quality between 21 and 30|
|__|| quality higher than 31|