Background, Leucoptera coffeella (Guerin-Meneville, 1842) is a moth species (Lepidoptera) pest which causes severe losses to the coffee crops. Further information about its genomic data is required to allow molecular strategies for the development of sustainable pesticides and to gain in-depth knowledge on phylogenetics. However, the closest complete genome available is within the superfamily level (Yponomeutoidea). Here, we report the sequencing and assembly of the nuclear genome of L. coffeella generated from Illumina and PacBio. Results, DNA sequencing from pupae samples resulted in 436,466,105,362 subreads and a total of 31,637,507 reads with mean contig N50 of 15,512 nt. The mean read length was 13.8 Kb nt and max read length was 420.7 Kb. Additionally, 20Gb data of short-insert paired-end (Novaseq6000 2x150 - Illumina) sequencing was used for higher resolution. The combined strategies produced 1,984 contigs comprising 397,153,904 nt in total size. The longest and shortest scaffold sizes are 10,809,567 nt and 15,247 nt, respectively (mean size 200,178 nt). The N50 scaffold was 275,598 nt and the GC content was 36.10%. Predicted coding DNA sequences count up to 39.930 gene models. Searching of 255 BUSCO groups revealed 97.6 percent of completeness (single and duplicated genes combined) compared to eukaryotic genomes (eukaryota_odb10). Flow cytometry showed the 1C DNA content is approximately 295 Mb. Conclusions, This first nuclear genome of the Lyonetiidae family brings valuable molecular resources to study other insect genomes and provides molecular tools towards sustainable biotechnology solutions for lepidopteran pests control.